Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 10(1): 183-191
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Nkadimeng Marble1,2*, van Marle-Köster2, Lekola Kgomotso4, Mphaphathi Masindi Lotus1, Lehloenya Khoboso Christina4, Pilane Cyril Mpho1, Nedambale Tshimangadzo Lucky1,3

1Agricultural Research Council, Animal Production Institute, Germplasm Conservation and Reproductive Biotechnologies, Private bag X2, Irene 0062, South Africa; 2Department of Animal and Wildlife Sciences, University of Pretoria, Private bag X20, Hatfield 0028, South Africa; 3Tshwane University of Technology, Faculty of Science, Department of Animal Sciences, Private bag X680; 4Department of Agriculture, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa.

Abstract | The study aimed to evaluate the effect of high temperature on cattle oocytes matured in vitro at 39, 41, 42, 43˚C and to evaluate apoptosis through Caspase-activity and DNA fragmentation on produced embryos. Cumulus oocyte complexes (COC) were matured at 39, 41, 42 and 43˚C. Two best maturation temperatures were selected for maturation throughout embryo production. Post maturation, matured oocytes were exposed to standard embryonic conditions. Embryos were evaluated at 3 stages: ≥ 2-4 cell, ≥ 8cell and blastocyst to determine Caspase-3 activity and DNA fragmentation. Maturation of oocytes at 41 and 39˚C demonstrated no significant difference (p>0.05) however, higher maturation rate compared to 42 and 43˚C. Matured oocytes at 39 and 41˚C showed no difference (p>0.05) in embryo development. Blastocysts at 39˚C maturation had higher (p<0.05) nuclei cells compared to those at 41˚C. Caspase-3 activity was comparable at ≥ 2-4 cell and ≥ 8cell for both 39 and 41˚C maturation temperatures, however, 41˚C showed high DNA fragmentation compared to 39˚C maturation group. Blastocyst from oocytes matured at 41˚C had higher Caspase-3 activity and DNA fragmentation than those at 39˚C. Embryos derived from 41˚C maturation temperature resulted in evidence of apoptosis compared to 39˚C.

Keywords | Oocyte maturation, High temperature, Embryos, Cell apoptosis, Blastocyst