Detection of virulence genes of C. pseudotuberculosis isolates from sheep and goats with CLA. Lane 1: 100 bp DNA marker, lane 2: amplification of 924 bp of pld, lane 3: amplification of fagA (245 bp), lane 4: amplification of 291 bp of fagB, lane 5: amplification of fagC (173 bp), lane 6: amplified product of fagD gene (226 bp). 

Phylogenetic tree of C. pseudotuberculosis isolates based on the rpoB gene sequence. The tree was conducted with MEGA7 using Neighbor-joining method and Bootstrap value of 1000 repetitions. Twenty two rpoB genes of the C. pseudotuberculosis isolates obtained in this study designated as C. pseudotuberculosis RA1-RA22 as shown in tree and they were deposited in the GenBank database under accessions number: MT974521 to MT974542).