Characteristic pock lesions on CAM of SPF-ECE after three blind successive passages. (Multiple small, opaque and circular).

Image of Sheep poxvirus particles visualized by transmission electron microscopy where white arrow points at the virus. SPV particle size ranges from 260-320nm, image A: magnification at X20.000 – B: magnification at X40.000 

Electrophoresis of the amplified product of PCR (570bp). L: 100 bp DNA marker, p: positive control, N: negative control, from 1 to 10 samples. 

Phylogenetic tree for ORF103 partial nucleotide sequence of sheep poxvirus strain (SPPV_AHRI_1) and (SPPV_AHRI_2) (Acc. No MT210507 and MT210508) (Red circle) compared with other reference strains in GenBank database. The scale bar represents the number of substitutions per nucleotide. 

Percentage of nucleotides identity between SPPV AHRI strains and SPPV reference strains published on GenBank based on partial sequence of ORF103.