PCR amplification for aflaR gene of A.flavus(at 800pb) Lane L:100 bp DNA ladder standard. Lane 1: Positive control of A.flavus. Lane 2-5 A.flavus isolated from mastitis 

The PCR amplification for stx1gene of E.coli O175(at 306pb).Lane R: 100bp DNA ladder standard; Lane 1: Positive control of E.coli 0157., Lane 2-5: the isolates from mastitis 

The PCR amplification for aflaR gene of A.flavus(at 800pb) Lane 1:100 bp DNA ladder standard. Lane 2: Positive control of A.flavus.Lane 3: Negative control (Fusarium sp.) Lane 4: Treated 100 μg /ml of ZnONPs).Lane5: Treated 500 μg /ml of ZnONPs). Lane 6: Treated 0.25% C.O. Lane 7: Treated 1% C.O. Lane 8: Combination treat. of 100 μg /ml of ZnONPs+1%C.O. Lane9: Combination treat. of 100 μg /ml of ZnONPs+ 0.25% C.O. 

The PCR amplification for stx1gene of E.coli O175(at 306pb).Lane1: 100bp DNA ladder standard; Lane 2: Positive control of E.coli 0157.Lane 3: Negative control (S.aureas) Lane 4: Treated 100 μg /ml of ZnONPs). Lane 5: Treated 500 μg /ml of ZnONPs). Lane 6: Treated 0.25% C.O. Lane 7: Treated 1% C.O.Lane 8: Combination treat. of 100 μg /ml of ZnONPs+ 1% C.O. Lane 9: Combination treat. of 100 μg /ml of ZnONPs+ 0.25% C.O.