Advances in Animal and Veterinary Sciences

Short Communication
Adv. Anim. Vet. Sci. 8(2): 157-160
Http://dx.doi.org/10.17582/journal.aavs/2020/8.2.157.160
View Full HTML
Download PDF

Herintha Coeto Neitzke-Abreu1,2*, Victor Bruno Duarte Vieira1, Ana Paula Stefanello da Silveira1, Leticia Surian Batalini2, Laísa Vieira Gnutzmann1, Silvana de Oliveira Castro2, Silvia Barbosa do Carmo3, Elisabete Friozi3, Manoel Sebastião da Costa Lima-Junior4

1Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal da Grande Dourados (UFGD), 79804-070, Dourados, MS, Brazil; 2Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados (UFGD), 79804-070, Dourados, MS, Brazil; 3Control Center of Zoonosis (CCZ), 79074-460, Campo Grande, MS, Brazil; 4Fundação Oswaldo Cruz (FIOCRUZ), Instituto Aggeu Magalhães, 50740-456, Recife, PE, Brazil.

Abstract | The objective of this study was to evaluate a polymerase chain reaction (PCR) for the diagnosis of Canine leishmaniasis (CanL) using less invasive sample collection. PCR-based detection of Leishmania infantum using ear peripheral blood collected from dogs with symptomatic CanL and coinfection with Ehrlichia sp. in the city of Campo Grande (Brazil) was investigated. A total of 268 CanL seropositive dogs were sampled. Of the 268 dogs tested, 70.90% were PCR positive for CanL, 72.43% of the dogs with ear lesion/s (185) were PCR positive, whereas only 67.47% of dogs without ear lesion/s (83) were PCR positive. Additionally, 35.82% of dogs tested were positive based on parasitological diagnosis for Ehrlichia sp. It was concluded that ear blood PCR may be an alternative for the diagnosis of CanL. Because it is more rapid collect and less invasive sample, this makes ear-blood PCR a potentially useful supplement to traditional diagnostic methods.

Keywords | Dog, Ehrlichia, Peripheral blood, Leishmania infantum, Co-infection