Time (s) to reach light sedation (A), light anesthesia (B) and deep anesthesia (C) stages at different concentrations of clove oil for tambacu juveniles (♂Piaractus mesopotamicus x ♀Colossoma macropomum). Values (mean ± SEM, n = 16) with different letters are significantly different between anesthetic concentrations. 

Partial recovery time (s) R2 (A) and total recovery R3 (B) at different concentrations of clove oil for tambacu juveniles (♂Piaractus mesopotamicus x ♀Colossoma macropomum). Values (mean ± SEM, n = 16) with different letters are significantly different between anesthetic agent concentrations. 

Relationship between deep anesthetic induction time (s) (A) and total recovery time (B) at different concentrations of clove oil for tambacu juveniles (♂Piaractus mesopotamicus x ♀Colossoma macropomum). Values are presented as mean ± standard error of the mean (SEM) (n = 16).